The 30th International Conference on Arabidopsis Research (ICAR 2019)

674 / 2019-04-30 16:28:23

In silico study on Bcl-2 athanogene expression in response to environmental stresses

BAG,ABA

Plenary track > Abiotic Stress

BAG (Bcl-2 athanogene) proteins are conserved in many eukaryotes and they have been suggested to play an essential role in plant cell program cell death (PCD). Seven homologs of the BAG gene have been identified in the Arabidopsis genome with the help of advanced bioinformatics tools during the past decade; These genes are involved in pathogenic attack and abiotic stress conditions. In this study, gene expression of members of the Arabidopsis BAG family under environmental stress was analyzed using Botany Array Resource (BAR) expression browser tool, and in silico data was partially confirmed by qRT-PCR analysis of selected stress- and hormone- treatment conditions related to Environmental stress. Especially, induction of AtBAG6 gene by heat shock was confirmed by using GUS reporter lines. The loss of the AtBAG6 gene by heat shock was confirmed by using GUS reporter lines. The loss of the AtBAG6 gene has impaired plant basal resistance and increased cell death in response to heat stress. To elucidate the regulatory mechanisms of BAG genes, analyzed ~1-kbp promoter regions for the presence of stress-responsive elements. Finally, our profiling results revealed that the Arabidopsis BAG gene response differentially to environmental stresses under the control of specifically organized upstream of regulatory elements.