The 30th International Conference on Arabidopsis Research (ICAR 2019)

694 / 2019-05-01 15:45:44

Functions of a pathogen-responsive protein, AtDabb1 with an antifungal activity in induced defense

Antifungal activity,AtDabb1,Arabidopsis,salicylic acid,jasmonate acid

Plenary track > Biotic Stress

Using anion exchange chromatography and high-performance liquid chromatography, we successfully isolated and purified a plant antifungal protein from Arabidopsis thaliana leaves. The amino acid sequence of the purified protein was identified by MALDI-TOF/MS analysis and that sequence corresponded with a hypothetical Arabidopsis protein in GenBank (accession number NP_175547). We designated the protein as AtDabb1. Full-length cDNA encoding the AtDabb1 gene was cloned from an Arabidopsis leaf cDNA library, the recombinant protein was expressed in Escherichia coli and found to significantly inhibit cell growth of various pathogenic fungal strains. mRNA expression of the AtDabb1 gene was induced by pathogen-related signaling molecules including salicylic acid (SA) and jasmonic acid (JA). These results suggest that AtDabb1 may play an important role in the induced defense pathway against diverse pathogenic fungi and various stresses.