Abstract Details
162 / 2024-06-11 18:49:34
Highly-Sensitive Detection and Quantification of Amyloid-β Aggregation in Alzheimer's Disease via Fluorescence Lifetime Imaging
Fluorescence Lifetime Imaging, Alzheimer's disease, Amyloid-beta, photobiomodulation
Draft Pending
Accurate detection and quantification of Amyloid-beta (Aβ) aggregation are crucial for understanding the mechanisms underlying Alzheimer's disease (AD). Despite advancements, conventional fluorescence imaging techniques still struggle to precisely monitor specific aggregation states. In this study, we developed NIR-Aβ, a polarity-sensitive fluorescent lifetime probe, to monitor Aβ protein aggregation dynamics during AD progression. Both in vitro and in vivo experiments confirm NIR-Aβ's Aβ-specific targeting, high polarity detection sensitivity, and excellent blood-brain barrier penetration. Using fluorescence lifetime imaging (FLIM) with NIR-Aβ, we successfully tracked Aβ aggregation dynamics in photobiomodulation experiments. Interestingly, we observed significant changes in Aβ aggregation fluorescence lifetime following photobiomodulation treatment, despite unchanged morphology, decreasing from 1.5 ns to 0.8 ns. These findings pave the way for accurately assessing and quantifying photobiomodulation therapy's impact on AD using FLIM, enhancing therapeutic evaluation and clinical outcome prediction.
Important Date
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Conference Date
Sep 08
2024
toSep 12
2024
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Sep 15 2024
Draft paper submission deadline
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Sep 15 2024
Registration deadline
Sponsored By
ShenZhen University
Contact Information
- Organizing Committee
- ic******@163.com
- +86********
Previous Conferences
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2009-05-11 China 哈尔滨市
The International Conference on Nanophotonics 2009
