The 11th International Symposium on 3D Genomics

36 / 2024-09-29 13:20:57

Natural Antibacterial Systems and Ubiquitination Regulation: Novel Therapeutic Targets and Disease-Resistant Breeding Strategies for Treating Dairy Animal Mastitis

Transcriptome; QTL; Mastitis; Ubiquitination

Dairy mastitis is a common and challenging disease that seriously affects the quality of dairy products and the economic benefits of animal husbandry. This study systematically analyzed transcriptome data from healthy and diseased milk animal breast samples in order to uncover the molecular mechanisms underlying mastitis pathogenesis and explore potential therapeutic and breeding strategies. Differential gene enrichment analysis showed that inflammatory response and immune regulation play a central role in the occurrence and development of mastitis. Differential genes were significantly enriched in gene body (GO) entries related to signaling pathways such as cytokine-cytokine receptor interactions, cell adhesion molecules, and JAK-STAT. Integrated quantitative trait locus (QTL) association analysis suggests that CXCR1/CXCR2 signaling pathway may be a potential therapeutic target for mastitis, and specific inhibitors of CXCR1 and CXCR2 are expected to be used in clinical treatment. In addition, the natural antibacterial system (OSCN-/HOSCN) mediated by endogenous high expression of LPO gene provides a new idea for breeding strategies for disease resistance. In conclusion, this study systematically explains the key molecular mechanisms of mastitis pathogenesis, lays a foundation for the future development of more effective clinical treatment of mastitis and the breeding of new strains, and is of great significance for promoting the sustainable development of animal husbandry.